Introduction to Sequence Manipulation:

• Sequence manipulation involves performing operations on biological sequences to extract useful information or transform the sequence data.
• Biopython provides a range of functionalities to manipulate DNA, RNA, and protein sequences.

The Seq Object:

• Biopython’s Seq object represents a biological sequence.
• It encapsulates the sequence data and provides various methods for sequence manipulation.

Sequence Extraction:

• Biopython allows extracting subsequences from larger sequences based on specified start and end positions.
• The extracted subsequence retains the same type (DNA, RNA, or protein) as the original sequence.

from Bio.Seq import Seq

sequence = Seq("ATCGATCGATCG")
subsequence = sequence[2:6]

print("Original Sequence:", sequence)
print("Subsequence:", subsequence)

• Create a Seq object from a DNA sequence.
• Extract a subsequence from position 2 to 5 (inclusive).
• Print both the original sequence and the extracted subsequence.

Reverse Complementation:

• Biopython provides a method to obtain the reverse complement of a DNA sequence.
• The reverse complement is useful for studying the complementary strand or designing primers.

from Bio.Seq import Seq

sequence = Seq("ATCGATCGATCG")
reverse_complement = sequence.reverse_complement()

print("Original Sequence:", sequence)
print("Reverse Complement:", reverse_complement)

• Create a Seq object from a DNA sequence.
• Use the reverse_complement() method to obtain the reverse complement.
• Print both the original sequence and the reverse complement.